GPC3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F49601
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49601-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49601-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the GPC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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GPC3 antibody western blot analysis in Jurkat lysate
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GPC3 antibody western blot analysis in mouse lung tissue lysate
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Immunofluorecence staining of GPC3 antibody on HepG2 cells. The cells were acetone fixated. Ab dilution of 1:50. Original magnification 1:400. (Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.)
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GPC3 antibody western blot analysis in Jurkat lysate
GPC3 antibody western blot analysis in mouse lung tissue lysate
Immunofluorecence staining of GPC3 antibody on HepG2 cells. The cells were acetone fixated. Ab dilution of 1:50. Original magnification 1:400. (Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.)

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000
Application Note:
Titration of the GPC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 529-560 from the human protein was used as the immunogen for this GPC3 antibody.
Limitation:
This GPC3 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P51654