HDAC1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F41123
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41123-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41123-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the HDAC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of HDAC1 antibody and HEK293 cells. Knockdown using siRNA showed a significant decrease of specific protein. Predicted molecular weight ~60 kDa
2 / 3
Fluorescent image of HeLa cell stained with HDAC1 antibody at 1:25. Immunoreactivity is localized to the nucleus.
3 / 3
Western blot testing of HDAC1 antibody at 1:2000 dilution. Lane 1: HeLa lysate; 2: Jurkat lysate; 3: K562 lysate; 4: mouse thymus lysate; Predicted molecular weight ~60 kDa
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Western blot testing of HDAC1 antibody and HEK293 cells. Knockdown using siRNA showed a significant decrease of specific protein. Predicted molecular weight ~60 kDa
Fluorescent image of HeLa cell stained with HDAC1 antibody at 1:25. Immunoreactivity is localized to the nucleus.
Western blot testing of HDAC1 antibody at 1:2000 dilution. Lane 1: HeLa lysate; 2: Jurkat lysate; 3: K562 lysate; 4: mouse thymus lysate; Predicted molecular weight ~60 kDa

Documents

Further Information

Application Details :
Western blot: 1:1000-2000,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the HDAC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HDAC1 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 449-482 from the human protein was used as the immunogen for this HDAC1 antibody.
Limitation:
This HDAC1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Mouse
Uniprot #:
Q13547