H-RAS Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F41616
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41616-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41616-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the H-RAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
H-RAS antibody western blot analysis in mouse cerebellum tissue lysate. Predicted molecular weight ~ 21 kDa.
2 / 3
Western blot analysis of H-RAS antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the HRAS gene.
3 / 3
Confocal immunofluorescent analysis of H-RAS antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
❮❯

H-RAS antibody western blot analysis in mouse cerebellum tissue lysate. Predicted molecular weight ~ 21 kDa.
Western blot analysis of H-RAS antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the HRAS gene.
Confocal immunofluorescent analysis of H-RAS antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the H-RAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 146-176 from the human protein was used as the immunogen for this H-RAS antibody.
Limitation:
This H-RAS antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Chicken
Uniprot #:
P01112