LC3B Antibody (MAP1LC3B)

NSJ Bioreagents
Product Code: NSJ-F46116
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F46116-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46116-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the LC3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of LC3B antibody and MCF-7 lysate
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LC3B antibody western blot analysis in mouse cerebellum tissue lysate
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LC3B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.
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LC3B antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Western blot analysis of LC3B antibody and MCF-7 lysate
LC3B antibody western blot analysis in mouse cerebellum tissue lysate
LC3B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.
LC3B antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 74-106 from the human protein was used as the immunogen for this LC3B antibody.
Limitation:
This LC3B antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q9GZQ8