AKT Antibody

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NSJ Bioreagents
Product Code: NSJ-F41829
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41829-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41829-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the AKT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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AKT antibody western blot analysis in MCF-7 lysate
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AKT antibody western blot analysis in mouse cerebellum tissue lysate
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AKT antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma
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Confocal immunofluorescent analysis of AKT antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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AKT antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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AKT antibody western blot analysis in MCF-7 lysate
AKT antibody western blot analysis in mouse cerebellum tissue lysate
AKT antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma
Confocal immunofluorescent analysis of AKT antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
AKT antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. [RefSeq].
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 428-457 from human AKT1 was used as the immunogen for this AKT antibody.
Limitation:
This AKT antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Bovine, Rat
Uniprot #:
P31749