ATG7 Antibody

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NSJ Bioreagents
Product Code: NSJ-F46229
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F46229-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46229-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Fluorescent image of Chloroquine treated U251 cells stained with ATG7 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
2 / 5
Western blot analysis of ATG7 antibody and 293 lysate transiently transfected with the human gene.
3 / 5
Western blot testing of ATG7 antibody and mouse liver tissue lysate. Predicted molecular weight: 70-80 kDa.
4 / 5
ATG7 antibody western blot analysis in HeLa lysate
5 / 5
IHC analysis of FFPE human hepatocarcinoma tissue stained with the ATG7 antibody
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Fluorescent image of Chloroquine treated U251 cells stained with ATG7 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Western blot analysis of ATG7 antibody and 293 lysate transiently transfected with the human gene.
Western blot testing of ATG7 antibody and mouse liver tissue lysate. Predicted molecular weight: 70-80 kDa.
ATG7 antibody western blot analysis in HeLa lysate
IHC analysis of FFPE human hepatocarcinoma tissue stained with the ATG7 antibody

Documents

Further Information

Application Details :
Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosomal/vacuolar system. ATG7 is a ubiquitin-activating enzyme E1-like protein essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. [OMIM]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 494-523 from the human protein was used as the immunogen for this ATG7 antibody.
Limitation:
This ATG7 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat
Uniprot #:
O95352