BNIP3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F42817
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42817-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42817-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the BNIP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Fluorescent confocal image of HepG2 cells stained with BNIP3 antibody at 1:50. BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
2 / 4
Freshly isolated mouse hepatocytes were cultured under normoxic conditions for 6 hr. and stained with BNIP3 antibody; Red: F-actin, Blue: ATP-synthase, Green: BNIP3. (Courtesy of Ruben Zamora, University of Pittsburgh.)
3 / 4
IHC analysis of FFPE human breast carcinoma tissue stained with the BNIP3 antibody
4 / 4
The BNIP3 antibody western blot analysis of Ramos cell lysate (lane 1) and in mouse brain tissue lysate (2).
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Fluorescent confocal image of HepG2 cells stained with BNIP3 antibody at 1:50. BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
Freshly isolated mouse hepatocytes were cultured under normoxic conditions for 6 hr. and stained with BNIP3 antibody; Red: F-actin, Blue: ATP-synthase, Green: BNIP3. (Courtesy of Ruben Zamora, University of Pittsburgh.)
IHC analysis of FFPE human breast carcinoma tissue stained with the BNIP3 antibody
The BNIP3 antibody western blot analysis of Ramos cell lysate (lane 1) and in mouse brain tissue lysate (2).

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:50-1:100,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the BNIP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 152-187 from the human protein (BH3 domain) was used as the immunogen for this BNIP3 antibody.
Limitation:
This BNIP3 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q12983