PDK4 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50055
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50055-0.05ML0.05 ml£260.00
Quantity:
NSJ-F50055-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PDK4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot of PDK4 antibody and mouse skeletal muscle tissue lysate. Predicted molecular weight ~46 kDa
2 / 4
Fluorescent confocal image of HeLa cells stained with PDK4 antibody. For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PDK4 is localized to the cytoplasm.
3 / 4
IHC analysis of FFPE human adrenal tissue stained with PDK4 antibody
4 / 4
IHC analysis of FFPE human skeletal muscle stained with PDK4 antibody
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Western blot of PDK4 antibody and mouse skeletal muscle tissue lysate. Predicted molecular weight ~46 kDa
Fluorescent confocal image of HeLa cells stained with PDK4 antibody. For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PDK4 is localized to the cytoplasm.
IHC analysis of FFPE human adrenal tissue stained with PDK4 antibody
IHC analysis of FFPE human skeletal muscle stained with PDK4 antibody

Documents

Further Information

Application Details :
Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the PDK4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 382-410 from the human protein was used as the immunogen for this PDK4 antibody.
Limitation:
This PDK4 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q16654