p-STAT3 Antibody (pS727)

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NSJ Bioreagents
Product Code: NSJ-F48455
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48455-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48455-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the p-STAT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of p-STAT3 antibody and mouse liver tissue/lysate collected before (-) or after (+) stimulation with IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
2 / 4
Fluorescent confocal image of SY5Y cells stained with p-STAT3 antibody at 1:200. Note the highly specific localization of the immunosignal to the nucleus.
3 / 4
Western blot testing of p-STAT3 antibody and HL-60 cells/lysate collected before (-) or after (+) stimulation with IFN-a.
4 / 4
IHC analysis of FFPE human breast carcinoma tissue stained with the p-STAT3 antibody.
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Western blot testing of p-STAT3 antibody and mouse liver tissue/lysate collected before (-) or after (+) stimulation with IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
Fluorescent confocal image of SY5Y cells stained with p-STAT3 antibody at 1:200. Note the highly specific localization of the immunosignal to the nucleus.
Western blot testing of p-STAT3 antibody and HL-60 cells/lysate collected before (-) or after (+) stimulation with IFN-a.
IHC analysis of FFPE human breast carcinoma tissue stained with the p-STAT3 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the p-STAT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This p-STAT3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS727 of human STAT3.
Limitation:
This phospho-p-STAT3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P40763