Vimentin Antibody

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NSJ Bioreagents
Product Code: NSJ-F48161
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48161-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48161-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Fluorescent confocal image of SY5Y cells stained with Vimentin antibody at 1:200. The immunosignal is localized to the cytoskeleton.
2 / 4
Western blot analysis of Vimentinin antibody and HeLa lysate
3 / 4
Western blot analysis of Vimentin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2).
4 / 4
IHC analysis of FFPE human breast carcinoma tissue stained with Vimentin antibody
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Fluorescent confocal image of SY5Y cells stained with Vimentin antibody at 1:200. The immunosignal is localized to the cytoskeleton.
Western blot analysis of Vimentinin antibody and HeLa lysate
Western blot analysis of Vimentin antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2).
IHC analysis of FFPE human breast carcinoma tissue stained with Vimentin antibody

Documents

Further Information

Application Details :
Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 63-90 from the human protein was used as the immunogen for this Vimentin antibody.
Limitation:
This Vimentin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Bovine, Hamster, Primate, Rat
Uniprot #:
P08670