LC3B Antibody (MAP1LC3B)

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NSJ Bioreagents
Product Code: NSJ-F46149
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F46149-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46149-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
Storage:
Aliquot the LC3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Mouse leukaemic monocyte macrophage cells treated with drug U18666A, causing cholesterol and lipid storage in cells, blocking fusion between late endosomes and lysosomes. LC3B antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures.
2 / 3
Fluorescent image of U251 cells stained with LC3B antibody at 1:100. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
3 / 3
SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Testing with LC3B antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
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Mouse leukaemic monocyte macrophage cells treated with drug U18666A, causing cholesterol and lipid storage in cells, blocking fusion between late endosomes and lysosomes. LC3B antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures.
Fluorescent image of U251 cells stained with LC3B antibody at 1:100. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Testing with LC3B antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:100,Immunocytochemistry: 1:10-1:50
Application Note:
Titration of the LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3B is one of the light chain subunits and can associate with either MAP1A or MAP1B. It is a Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 89-122 from the human protein was used as the immunogen for this LC3B antibody.
Limitation:
This LC3B antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Bovine
Uniprot #:
Q9GZQ8