SYVN1 Antibody (HRD1)

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NSJ Bioreagents
Product Code: NSJ-F40119
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40119-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40119-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SYVN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Fluorescent confocal image of HeLa cells stained with SYVN1 antibody. Primary Ab (1:200, 2 h at room temperature); Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used as secondary (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
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Western blot testing of SYVN1 antibody and transiently transfected Neuro2A cells
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Western blot analysis of SYVN1 antibody in T47D lysate
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Western blot analysis of SYVN1 mouse kidney tissue lysate
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IHC analysis of FFPE human breast carcinoma tissue stained with the SYVN1 antibody
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IHC analysis of FFPE human liver tissue stained with SYVN1 antibody
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Fluorescent confocal image of HeLa cells stained with SYVN1 antibody. Primary Ab (1:200, 2 h at room temperature); Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used as secondary (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
Western blot testing of SYVN1 antibody and transiently transfected Neuro2A cells
Western blot analysis of SYVN1 antibody in T47D lysate
Western blot analysis of SYVN1 mouse kidney tissue lysate
IHC analysis of FFPE human breast carcinoma tissue stained with the SYVN1 antibody
IHC analysis of FFPE human liver tissue stained with SYVN1 antibody

Documents

Further Information

Application Details :
Western blot: 1:200-1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the SYVN1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HRD1/SYVN1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of SYVN1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. It is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 586-617 from the human protein was used as the immunogen for this SYVN1 antibody.
Limitation:
This SYVN1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q86TM6