ALK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50788
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50788-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50788-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ALK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot of ALK1 antibody and mouse heart tissue lysate. Predicted molecular weight ~60 kDa.
2 / 5
Western blot analysis of ALK1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (2).
3 / 5
Western blot testing of human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ALK1 (2, 4); ALK1 antibody used at 1:1000, blocking solution is 5% milk in TBST (lane 1 and 2), and 5% BSA in TBST (3 and 4). Data courtesy of Kenneth Finnson, Montreal General Hospital.
4 / 5
IHC analysis of FFPE human hepatocarcinoma tissue stained with the ALK1 antibody
5 / 5
Flow cytometric analysis of HepG2 cells using ALK1 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot of ALK1 antibody and mouse heart tissue lysate. Predicted molecular weight ~60 kDa.
Western blot analysis of ALK1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (2).
Western blot testing of human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ALK1 (2, 4); ALK1 antibody used at 1:1000, blocking solution is 5% milk in TBST (lane 1 and 2), and 5% BSA in TBST (3 and 4). Data courtesy of Kenneth Finnson, Montreal General Hospital.
IHC analysis of FFPE human hepatocarcinoma tissue stained with the ALK1 antibody
Flow cytometric analysis of HepG2 cells using ALK1 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:50-1:100,Western blot: 1:1000
Application Note:
Titration of the ALK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ACVRL1 is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. This protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 38-68 from the human protein was used as the immunogen for this ALK1 antibody.
Limitation:
This ALK1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P37023