ROR1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50667
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50667-0.05ML0.05 ml£260.00
Quantity:
NSJ-F50667-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the ROR1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human samples 1) K562 and 2) lung lysate and mouse samples 3) kidney and 4) heart lysate with ROR1 antibody at 1:1000. Predicted molecular weight of ROR1 isoforms: 105 kDa and 130 kDa.
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Western blot testing of human samples 1) K562 cell line, 2) MDA-MB-231, 3) PANC-1, 4) NCI-H226, 5) fetal lung and mouse samples 6) NIH3T3 and 7) heart lysate with ROR1 antibody at 1:1000. Predicted molecular weight of ROR1 isoforms: 105 kDa and 130 kDa.
3 / 9
Flow cytometric analysis of A549 cells using ROR1 antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
4 / 9
Immunohistochemical analysis of paraffin-embedded human heart section using ROR1 antibody. Ab was diluted at 1:25 dilution.
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IHC testing of FFPE human lung carcinoma with ROR1 antibody.
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IHC testing of FFPE human kidney tissue with ROR1 antibody at 1:25 dilution. HIER: steamed antigen retrieval with pH6 citrate buffer.
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IHC testing of FFPE human kidney tissue with ROR1 antibody at 1:100 dilution. HIER: steamed antigen retrieval with pH9 EDTA buffer.
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IHC testing of FFPE human heart tissue with ROR1 antibody at 1:100 dilution. HIER: steamed antigen retrieval with pH9 EDTA buffer.
9 / 9
Western blot testing of human lysate samples 1) K562, 2) MDA-MB-231, 3) NCI-H226, 4) A549 and mouse samples 5) NIH3T3 and 6) kidney lysate with ROR1 antibody at 1:4000. Predicted molecular weight of ROR1 isoforms: 105 kDa and 130 kDa.
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Western blot testing of human samples 1) K562 and 2) lung lysate and mouse samples 3) kidney and 4) heart lysate with ROR1 antibody at 1:1000. Predicted molecular weight of ROR1 isoforms: 105 kDa and 130 kDa.
Western blot testing of human samples 1) K562 cell line, 2) MDA-MB-231, 3) PANC-1, 4) NCI-H226, 5) fetal lung and mouse samples 6) NIH3T3 and 7) heart lysate with ROR1 antibody at 1:1000. Predicted molecular weight of ROR1 isoforms: 105 kDa and 130 kDa.
Flow cytometric analysis of A549 cells using ROR1 antibody (green) compared to an isotype control of rabbit IgG (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
Immunohistochemical analysis of paraffin-embedded human heart section using ROR1 antibody. Ab was diluted at 1:25 dilution.
IHC testing of FFPE human lung carcinoma with ROR1 antibody.
IHC testing of FFPE human kidney tissue with ROR1 antibody at 1:25 dilution. HIER: steamed antigen retrieval with pH6 citrate buffer.
IHC testing of FFPE human kidney tissue with ROR1 antibody at 1:100 dilution. HIER: steamed antigen retrieval with pH9 EDTA buffer.
IHC testing of FFPE human heart tissue with ROR1 antibody at 1:100 dilution. HIER: steamed antigen retrieval with pH9 EDTA buffer.
Western blot testing of human lysate samples 1) K562, 2) MDA-MB-231, 3) NCI-H226, 4) A549 and mouse samples 5) NIH3T3 and 6) kidney lysate with ROR1 antibody at 1:4000. Predicted molecular weight of ROR1 isoforms: 105 kDa and 130 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000-2000,Flow Cytometry: 1:25,IHC (Paraffin): 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ROR1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This ROR1 antibody was produced from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).
Limitation:
This ROR1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q01973