KLF4 Antibody

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NSJ Bioreagents
Product Code: NSJ-F48145
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48145-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48145-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human spleen tissue stained with KLF4 antibody
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IHC testing of KLF4 antibody and FFPE human prostate carcinoma
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IF staining of HeLa cells with KLF4 antibody at 1:100, response is localized to the nuclei.
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Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:100. KLF4 immunoreactivity is localized to the nuclei.
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KLF4 antibody western blot with (1) Jurkat, (2) 293 lysate. Predicted molecular weight: 50-60 kDa + ~75 kDa
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IHC analysis of FFPE human spleen tissue stained with KLF4 antibody
IHC testing of KLF4 antibody and FFPE human prostate carcinoma
IF staining of HeLa cells with KLF4 antibody at 1:100, response is localized to the nuclei.
Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:100. KLF4 immunoreactivity is localized to the nuclei.
KLF4 antibody western blot with (1) Jurkat, (2) 293 lysate. Predicted molecular weight: 50-60 kDa + ~75 kDa

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development Contributes to the down-regulation of p53/TP53 transcription.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 69-101 from the human protein was used as the immunogen for this KLF4 antibody.
Limitation:
This KLF4 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
O43474