IL-4 Antibody

NSJ Bioreagents
Product Code: NSJ-F49086
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49086-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49086-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the IL-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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(Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot analysis of IL-4 antibody and mouse cerebellum lysate
2 / 3
IL-4 antibody IHC analysis in formalin fixed and paraffin embedded tonsil
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IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.-

(Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot analysis of IL-4 antibody and mouse cerebellum lysate
IL-4 antibody IHC analysis in formalin fixed and paraffin embedded tonsil
IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.-

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 122-150 from the human protein was used as the immunogen for this IL-4 antibody.
Limitation:
This IL-4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Primate
Uniprot #:
P05112