PHGDH Antibody

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NSJ Bioreagents
Product Code: NSJ-F48338
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48338-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48338-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2). Predicted molecular weight ~56 kDa.
2 / 4
Western blot analysis of PHGDH antibody and mouse brain tissue lysate. Predicted molecular weight ~56 kDa.
3 / 4
IHC analysis of FFPE human brain tissue stained with PHGDH antibody
4 / 4
Flow cytometric analysis of HeLa cells using PHGDH antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2). Predicted molecular weight ~56 kDa.
Western blot analysis of PHGDH antibody and mouse brain tissue lysate. Predicted molecular weight ~56 kDa.
IHC analysis of FFPE human brain tissue stained with PHGDH antibody
Flow cytometric analysis of HeLa cells using PHGDH antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 140-168 from the human protein was used as the immunogen for this PHGDH antibody.
Limitation:
This PHGDH antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Bovine, Pig, Primate
Uniprot #:
O43175