ASS1 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F49896
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49896-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49896-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ASS1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot testing of human 1) A431, 2) MOLT4, 3) Jurkat, 4) PC-12, 5) mouse NIH3T3 and 6) mouse brain lysate with ASS1 antibody at 1:2000. Predicted molecular weight ~46 kDa.
2 / 6
Confocal immunofluorescent analysis of ASS1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
3 / 6
Immunofluorescent staining of permeabilized human HeLa cells with ASS1 antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-rabbit lgG (green). Counterstains: blue (nuclear) and red (actin).
4 / 6
IHC testing of FFPE human cervical cancer with ASS1 antibody at 1:25 dilution. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
IHC testing of FFPE human kidney with ASS1 antibody at 1:25 dilution. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
6 / 6
Flow cytometric analysis of WiDr cells using ASS1 antibody (green) compared to a?negative control?(blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
❮❯

Western blot testing of human 1) A431, 2) MOLT4, 3) Jurkat, 4) PC-12, 5) mouse NIH3T3 and 6) mouse brain lysate with ASS1 antibody at 1:2000. Predicted molecular weight ~46 kDa.
Confocal immunofluorescent analysis of ASS1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Immunofluorescent staining of permeabilized human HeLa cells with ASS1 antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-rabbit lgG (green). Counterstains: blue (nuclear) and red (actin).
IHC testing of FFPE human cervical cancer with ASS1 antibody at 1:25 dilution. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human kidney with ASS1 antibody at 1:25 dilution. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometric analysis of WiDr cells using ASS1 antibody (green) compared to a?negative control?(blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the ASS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ASS catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 192-221 from the human protein was used as the immunogen for this ASS1 antibody.
Limitation:
This ASS1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat
Uniprot #:
P00966