APG7 Antibody

Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Titration of the APG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 284-313 from the human protein was used as the immunogen for this APG7 antibody.

This APG7 antibody is available for research use only.

Purified

Human, Mouse

Rat, Chicken

O95352