PRKR Antibody

NSJ Bioreagents
Product Code: NSJ-F40175
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40175-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40175-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PRKR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
PRKR antibody used in western blot to detect PRKR/PKR in mouse uterus tissue lysate (Lane 1) and HepG2 cell lysate (2).
2 / 3
Western blot analysis of PRKR antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2/PKR gene (2).
3 / 3
IHC analysis of FFPE human hepatocarcinoma stained with the PRKR antibody

PRKR antibody used in western blot to detect PRKR/PKR in mouse uterus tissue lysate (Lane 1) and HepG2 cell lysate (2).
Western blot analysis of PRKR antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2/PKR gene (2).
IHC analysis of FFPE human hepatocarcinoma stained with the PRKR antibody

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PRKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Interferon-induced, double-stranded RNA-activated protein kinase is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PRKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. Human gamma-interferon (IFNG) mRNA exploits localized activation of PRKR in the cell to regulate its own translation. IFNG mRNA activates PRKR through a pseudoknot in its 5-prime untranslated region. The HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PRKR and the translation initiation factor EIF2-alpha, a target of PRKR. E2 inhibits the kinase activity of PRKR and blocks its inhibitory effect on protein synthesis and cell growth, which provides one mechanism by which HCV may circumvent the antiviral effect of interferon.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 519-550 from the human protein was used as the immunogen for this PRKR antibody.
Limitation:
This PRKR antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P19525