EZH2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F48073
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48073-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48073-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the EZH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of EZH2 antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: MDA-MB-231 lysate; 3: MDA-MB-468 lysate; 4: T47D lysate; 5: mouse spleen lysate; Predicted size: 85-95 kDa
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Western blot analysis of lysate from (1) MDA-MB-468, (2) SW620, (3) T47D cell line, (4) mouse spleen, (5) mouse testis tissue using EZH2 antibody at 1:1000. Predicted size: 85-95 kDa
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Flow cytometric analysis of HeLa cells using EZH2 antibody (green) compared to an <a href=search_result.php?search_txt=n1001>isotype control of rabbit IgG</a> (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
4 / 7
Formalin-fixed and paraffin-embedded human testis tissue tested with EZH2 antibody
5 / 7
Confocal immunofluorescent analysis of EZH2 antibody and 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
6 / 7
EZH2 antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to negative control cells (top histogram).
7 / 7
Confocal immunofluorescent analysis of EZH2 antibody and human HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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Western blot testing of EZH2 antibody at 1:2000 dilution. Lane 1: Jurkat lysate; 2: MDA-MB-231 lysate; 3: MDA-MB-468 lysate; 4: T47D lysate; 5: mouse spleen lysate; Predicted size: 85-95 kDa
Western blot analysis of lysate from (1) MDA-MB-468, (2) SW620, (3) T47D cell line, (4) mouse spleen, (5) mouse testis tissue using EZH2 antibody at 1:1000. Predicted size: 85-95 kDa
Flow cytometric analysis of HeLa cells using EZH2 antibody (green) compared to an <a href=search_result.php?search_txt=n1001>isotype control of rabbit IgG</a> (blue). Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.
Formalin-fixed and paraffin-embedded human testis tissue tested with EZH2 antibody
Confocal immunofluorescent analysis of EZH2 antibody and 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
EZH2 antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to negative control cells (top histogram).
Confocal immunofluorescent analysis of EZH2 antibody and human HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Documents

Further Information

Application Details :
Flow Cytometry: 1:25,Western blot: 1:1000,Immunohistochemistry (FFPE): 1:10-1:50,Immunofluorescence/Immunocytochemistry: 1:25
Application Note:
Titration of the EZH2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EZH2 is a protein that transfers sulfate to the C-4 hydroxyl of beta-1,4-linked GalNAc flanked by GlcUA residues in chondroitin.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This EZH2 antibody was produced from rabbits immunized with a recombinant protein from human EZH2.
Limitation:
This EZH2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q15910