ID1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F41649
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41649-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41649-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the ID1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from HepG2, MCF-7, U-2OS cell line (left to right) using ID1 antibody; Ab was diluted at 1:1000 for each lane.
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ID1 antibody western blot analysis in U251 lysate
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ID1 antibody western blot analysis in mouse heart tissue lysate
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ID1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue.
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Confocal immunofluorescent analysis of ID1 antibody with U-251MG cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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ID1 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of lysate from HepG2, MCF-7, U-2OS cell line (left to right) using ID1 antibody; Ab was diluted at 1:1000 for each lane.
ID1 antibody western blot analysis in U251 lysate
ID1 antibody western blot analysis in mouse heart tissue lysate
ID1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue.
Confocal immunofluorescent analysis of ID1 antibody with U-251MG cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
ID1 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the ID1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 66-93 from the human protein was used as the immunogen for this ID1 antibody.
Limitation:
This ID1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat
Uniprot #:
P41134