PGP9.5 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F47951
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F47951-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47951-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PGP9.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC analysis of FFPE human brain tissue stained with PGP9.5 antibody.
2 / 5
Western blot analysis of PGP9.5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (2).
3 / 5
Western blot analysis of PGP9.5 antibody and CEM (lane 1), Jurkat (2), Y79 (3) cell lines and mouse brain tissue (4) lysate. Predicted molecular weight ~25 kDa.
4 / 5
Confocal immunofluorescent analysis of PGP9.5 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
5 / 5
PGP9.5 antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
❮❯

IHC analysis of FFPE human brain tissue stained with PGP9.5 antibody.
Western blot analysis of PGP9.5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (2).
Western blot analysis of PGP9.5 antibody and CEM (lane 1), Jurkat (2), Y79 (3) cell lines and mouse brain tissue (4) lysate. Predicted molecular weight ~25 kDa.
Confocal immunofluorescent analysis of PGP9.5 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
PGP9.5 antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PGP9.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PGP9.5 is a Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 16-46 from the human protein was used as the immunogen for this PGP9.5 antibody.
Limitation:
This PGP9.5 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Bovine, Pig
Uniprot #:
P09936