Anti-ATG5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F46219
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F46219-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46219-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the anti-ATG5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Fluorescent image of U251 cells stained with anti-ATG5 antibody diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
2 / 3
Western blot analysis of anti-ATG5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
3 / 3
IHC analysis of FFPE human hepatocarcinoma tissue stained with the anti-ATG5 antibody
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Fluorescent image of U251 cells stained with anti-ATG5 antibody diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
Western blot analysis of anti-ATG5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
IHC analysis of FFPE human hepatocarcinoma tissue stained with the anti-ATG5 antibody

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the anti-ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 209-238 from the human protein was used as the immunogen for this anti-ATG5 antibody.
Limitation:
This anti-ATG5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q9H1Y0