IRF9 Antibody

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NSJ Bioreagents
Product Code: NSJ-F41756
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41756-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41756-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the IRF9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of IRF9 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
2 / 4
IRF9 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue.
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IRF9 antibody western blot analysis in mouse spleen tissue lysate.
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IRF9 antibody western blot analysis in mouse NIH3T3 cell lysate. Predicted molecular weight: 44-48 kDa.
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Confocal immunofluorescent analysis of IRF9 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IRF9 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue.
IRF9 antibody western blot analysis in mouse spleen tissue lysate.
IRF9 antibody western blot analysis in mouse NIH3T3 cell lysate. Predicted molecular weight: 44-48 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the IRF9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IRF9 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 75-104 from the human protein was used as the immunogen for this IRF9 antibody.
Limitation:
This IRF9 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q00978