LSD1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F41978
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F41978-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41978-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the LSD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of LSD1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the AOF2/LSD1 gene (2).
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Western blot analysis of LSD1 antibody and mouse brain tissue lysate.
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IHC analysis of FFPE human breast carcinoma tissue stained with the LSD1 antibody
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Fluorescent confocal image of HeLa cell stained with LSD1 antibody. LSD1 immunoreactivity is localized to the cytoplasm strongly and nucleus weakly.
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Western blot analysis of LSD1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the AOF2/LSD1 gene (2).
Western blot analysis of LSD1 antibody and mouse brain tissue lysate.
IHC analysis of FFPE human breast carcinoma tissue stained with the LSD1 antibody
Fluorescent confocal image of HeLa cell stained with LSD1 antibody. LSD1 immunoreactivity is localized to the cytoplasm strongly and nucleus weakly.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the LSD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 108-142 from the human protein was used as the immunogen for this LSD1 antibody.
Limitation:
This LSD1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
O60341