SLUG Antibody (SNAI2)

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NSJ Bioreagents
Product Code: NSJ-F47911
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F47911-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47911-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SLUG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) mouse heart and 2) rat PC-12 lysate with SLUG antibody. Predicted molecular weight ~30 kDa.
2 / 4
Confocal immunofluorescent analysis of SLUG antibody with human HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
3 / 4
IHC analysis of FFPE human breast tissue stained with SLUG antibody.
4 / 4
Western blot testing of SLUG antibody and mouse heart lysate. Predicted molecular weight ~30 kDa.
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Western blot testing of 1) mouse heart and 2) rat PC-12 lysate with SLUG antibody. Predicted molecular weight ~30 kDa.
Confocal immunofluorescent analysis of SLUG antibody with human HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human breast tissue stained with SLUG antibody.
Western blot testing of SLUG antibody and mouse heart lysate. Predicted molecular weight ~30 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the SLUG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SLUG/SNAI2 is a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress?E-cadherin?transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporadic cases of neural tube defects. [Wiki]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 98-129 from human SNAI2 was used as the immunogen for this SLUG antibody.
Limitation:
This SLUG antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Bovine
Uniprot #:
O43623