Cleaved LC3A Antibody (ATG8a/APG8a)

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NSJ Bioreagents
Product Code: NSJ-F46138
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F46138-0.08ML0.08 ml£260.00
Quantity:
NSJ-F46138-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Cleaved LC3A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of human A431 cells, untreated or treated with chloroquine (100ng/ml) tested with Cleaved LC3A antibody or beta-Actin Ab.
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Western blot analysis of mouse NIH3T3 cells, untreated or treated with chloroquine (100ng/ml) tested with Cleaved LC3A antibody or beta-Actin Ab.
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Fluorescent image of U251 cells stained with Cleaved LC3A antibody (1:100). Immunoreactivity is localized to the autophagic vacuoles in the cytoplasm.
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Western blot analysis of human A431 cells, untreated or treated with chloroquine (100ng/ml) tested with Cleaved LC3A antibody or beta-Actin Ab.
Western blot analysis of mouse NIH3T3 cells, untreated or treated with chloroquine (100ng/ml) tested with Cleaved LC3A antibody or beta-Actin Ab.
Fluorescent image of U251 cells stained with Cleaved LC3A antibody (1:100). Immunoreactivity is localized to the autophagic vacuoles in the cytoplasm.

Documents

Further Information

Application Details :
Western blot: 1:500,Immunofluorescence: 1:100
Application Note:
Titration of the Cleaved LC3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3A/ATG8A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 89-120 from the human protein was used as the immunogen for this ATG8 / Cleaved LC3A antibody.
Limitation:
This Cleaved LC3A antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Bovine
Uniprot #:
Q9H492