IDH Antibody

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NSJ Bioreagents
Product Code: NSJ-F50421
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50421-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50421-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the IDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of IDH antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI nuclear counterstain (blue).
2 / 4
IHC analysis of FFPE human brain stained with IDH antibody
3 / 4
Western blot analysis of IDH antibody and mouse liver tissue and 293 lysate. Predicted molecular weight ~46 kDa.
4 / 4
Flow cytometric analysis of 293 cells using IDH antibody (bottom histogram) compared to a negative control (top histogram).
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Confocal immunofluorescent analysis of IDH antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI nuclear counterstain (blue).
IHC analysis of FFPE human brain stained with IDH antibody
Western blot analysis of IDH antibody and mouse liver tissue and 293 lysate. Predicted molecular weight ~46 kDa.
Flow cytometric analysis of 293 cells using IDH antibody (bottom histogram) compared to a negative control (top histogram).

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the IDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein is the NADP(+)-dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions. [Wiki]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 63-90 from the human protein was used as the immunogen for this IDH antibody.
Limitation:
This IDH antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Bovine
Uniprot #:
O75874