p73 Antibody

NSJ Bioreagents
Product Code: NSJ-F51407
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F51407-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51407-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the p73 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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p73 antibody western blot analysis in 1) human K562 cell line and 2) mouse brain tissue lysate.
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p73 antibody IHC analysis in formalin fixed and paraffin embedded mouse brain.
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Confocal immunofluorescent analysis of p73 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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p73 antibody flow cytometric analysis of 293 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

p73 antibody western blot analysis in 1) human K562 cell line and 2) mouse brain tissue lysate.
p73 antibody IHC analysis in formalin fixed and paraffin embedded mouse brain.
Confocal immunofluorescent analysis of p73 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
p73 antibody flow cytometric analysis of 293 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the p73 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TP73 is tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 288-317 from the human protein was used as the immunogen for this p73 antibody.
Limitation:
This p73 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
O15350