PGP9.5 Antibody

NSJ Bioreagents
Product Code: NSJ-F47952
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F47952-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47952-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PGP9.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
PGP9.5 antibody western blot analysis with (1) U266, (2) SH-SY5Y, (3) NCI-H1299, (4) mouse Neuro-2a, (5) rat C6, (6) PC-12 cell line and (7) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.
2 / 4
IHC analysis of FFPE human breast carcinoma tissue stained with the PGP9.5 antibody
3 / 4
PGP9.5 antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
4 / 4
Confocal immunofluorescent analysis of PGP9.5 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

PGP9.5 antibody western blot analysis with (1) U266, (2) SH-SY5Y, (3) NCI-H1299, (4) mouse Neuro-2a, (5) rat C6, (6) PC-12 cell line and (7) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.
IHC analysis of FFPE human breast carcinoma tissue stained with the PGP9.5 antibody
PGP9.5 antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of PGP9.5 antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the PGP9.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 187-216 from the human protein was used as the immunogen for this PGP9.5 antibody.
Limitation:
This PGP9.5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Bovine, Pig, Primate
Uniprot #:
P09936