MCL1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F42742
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42742-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42742-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the MCL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human breast carcinoma with MCL1 antibody
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Western blot analysis of MCL1 antibody and Ramos lysate. Predicted molecular weight: 37/29 kDa (isoforms 1/2).
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Flow cytometric analysis of ZR-75-1 cells using MCL1 antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC analysis of FFPE human breast carcinoma with MCL1 antibody
Western blot analysis of MCL1 antibody and Ramos lysate. Predicted molecular weight: 37/29 kDa (isoforms 1/2).
Flow cytometric analysis of ZR-75-1 cells using MCL1 antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the MCL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The Mcl-1 protein belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 191-226 from the human protein was used as the immunogen for this MCL1 antibody.
Limitation:
This MCL1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q07820