S100B Antibody

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NSJ Bioreagents
Product Code: NSJ-F49008
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49008-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49008-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the S100B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from (1) A431 cell line, (2) mouse brain and (3) rat brain tissue using S100B antibody at 1:1000. Predicted size: 11 kDa with a 21 kDa homodimer
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S100B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma.
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S100B antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of lysate from (1) A431 cell line, (2) mouse brain and (3) rat brain tissue using S100B antibody at 1:1000. Predicted size: 11 kDa with a 21 kDa homodimer
S100B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma.
S100B antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the S100B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This S100B antibody was produced from rabbits immunized with S100B recombinant protein.
Limitation:
This S100B antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rabbit
Uniprot #:
P04271