FGFR1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50611
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50611-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50611-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the FGFR1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 7E3 and FGFR1 antibody. FGFR is visualized as red fluorescence (i,iv,vii), A1B3 is visualized as green fluorescence (ii,v,viii), and colocalization of FGF2 and fibrinogen receptors is shown as yellow fluorescence (iii,vi,ix). Insets represent the background fluorescence.
2 / 4
Flow cytometric analysis of MCF-7 cells using FGFR1 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
3 / 4
IHC analysis of FFPE human breast carcinoma tissue stained with the FGFR1 antibody
4 / 4
The FGFR1 antibody used in western blot to detect FGFR1 in NIH3T3 cell lysate. Predicted molecular weight: 75-160 kDa depending on glycosylation level.
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Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 7E3 and FGFR1 antibody. FGFR is visualized as red fluorescence (i,iv,vii), A1B3 is visualized as green fluorescence (ii,v,viii), and colocalization of FGF2 and fibrinogen receptors is shown as yellow fluorescence (iii,vi,ix). Insets represent the background fluorescence.
Flow cytometric analysis of MCF-7 cells using FGFR1 antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human breast carcinoma tissue stained with the FGFR1 antibody
The FGFR1 antibody used in western blot to detect FGFR1 in NIH3T3 cell lysate. Predicted molecular weight: 75-160 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Flow Cytometry: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:50-1:100
Application Note:
Titration of the FGFR1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene can lead to Pfeiffer syndrome and Jackson-Weiss syndrome. The genomic organization of the gene is very similar to family members 2-4, encompassing 19 exons that are subject to complex alternative splicing, which allows for structural, tissue expression and ligand affinity variations among the isoforms.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 19-48 from the human protein was used as the immunogen for this FGFR1 antibody.
Limitation:
This FGFR1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P11362