PGK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50105
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50105-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50105-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PGK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC analysis of FFPE human kidney section using PGK1 antibody; Ab was diluted at 1:100.
2 / 5
Western blot analysis of lysate from HeLa, U-937 cell line (left to right) using PGK1 antibody; Ab was diluted at 1:1000 for each lane.
3 / 5
IHC analysis of FFPE human hepatocarcinoma tissue stained with PGK1 antibody
4 / 5
Confocal immunofluorescent analysis of PGK1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
5 / 5
PGK1 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC analysis of FFPE human kidney section using PGK1 antibody; Ab was diluted at 1:100.
Western blot analysis of lysate from HeLa, U-937 cell line (left to right) using PGK1 antibody; Ab was diluted at 1:1000 for each lane.
IHC analysis of FFPE human hepatocarcinoma tissue stained with PGK1 antibody
Confocal immunofluorescent analysis of PGK1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
PGK1 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 117-145 from the human protein was used as the immunogen for this PGK1 antibody.
Limitation:
This PGK1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Primate, Rat
Uniprot #:
P00558