SMAD3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F52046
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52046-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52046-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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SMAD3 antibody western blot analysis in HepG2 and mouse C2C12 lysate. Observed molecular weight: 48~55 kDa.
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SMAD3 antibody IHC analysis in formalin fixed and paraffin embedded brain tissue.
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Confocal immunofluorescent analysis of SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
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SMAD3 antibody flow cytometric analysis of MDA-MB231 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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SMAD3 antibody western blot analysis in HepG2 and mouse C2C12 lysate. Observed molecular weight: 48~55 kDa.
SMAD3 antibody IHC analysis in formalin fixed and paraffin embedded brain tissue.
Confocal immunofluorescent analysis of SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
SMAD3 antibody flow cytometric analysis of MDA-MB231 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 186-215 from the human protein was used as the immunogen for this SMAD3 antibody.
Limitation:
This SMAD3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Pig, Chicken
Uniprot #:
P84022