IDH1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50422
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50422-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50422-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the IDH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from (1) HepG2, (2) MCF-7 cell line, (3) human liver and (4) rat liver tissue using IDH1 antibody at 1:1000. Predicted molecular weight ~46 kDa.
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Western blot analysis of IDH1 antibody and HepG2 cell line and mouse liver tissue lysate. Predicted molecular weight ~46 kDa.
3 / 6
Confocal immunofluorescent analysis of IDH1 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
4 / 6
IHC analysis of FFPE human prostate section using IDH1 antibody; Ab was diluted at 1:100.
5 / 6
IHC analysis of FFPE human hepatocarcinoma tissue stained with IDH1 antibody
6 / 6
IDH1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of lysate from (1) HepG2, (2) MCF-7 cell line, (3) human liver and (4) rat liver tissue using IDH1 antibody at 1:1000. Predicted molecular weight ~46 kDa.
Western blot analysis of IDH1 antibody and HepG2 cell line and mouse liver tissue lysate. Predicted molecular weight ~46 kDa.
Confocal immunofluorescent analysis of IDH1 antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human prostate section using IDH1 antibody; Ab was diluted at 1:100.
IHC analysis of FFPE human hepatocarcinoma tissue stained with IDH1 antibody
IDH1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the IDH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [Wiki]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 116-143 from the human protein was used as the immunogen for this IDH1 antibody.
Limitation:
This IDH1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Bovine
Uniprot #:
O75874