Caspase-9 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50930
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50930-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50930-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Caspase-9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Caspase-9 antibody western blot analysis in 1) human HeLa and 2) mouse L929 lysate
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IHC analysis of FFPE human lung carcinoma tissue stained with Caspase-9 antibody
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Confocal immunofluorescent analysis of Caspase-9 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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Caspase-9 antibody western blot analysis in 1) human HeLa and 2) mouse L929 lysate
IHC analysis of FFPE human lung carcinoma tissue stained with Caspase-9 antibody
Confocal immunofluorescent analysis of Caspase-9 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the Caspase-9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 183-211 from the human protein was used as the immunogen for this Caspase-9 antibody.
Limitation:
This Caspase-9 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P55211