c-Src Antibody

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NSJ Bioreagents
Product Code: NSJ-F50715
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50715-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50715-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the c-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from (1) human MCF-7, (2) rat PC-12, and (3) mouse C2C12 cell line using c-Src antibody at 1:1000.
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IHC analysis of FFPE human breast carcinoma tissue stained with the c-Src antibody
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Confocal immunofluorescent analysis of c-Src antibody with A375 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
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Western blot analysis of lysate from (1) human MCF-7, (2) rat PC-12, and (3) mouse C2C12 cell line using c-Src antibody at 1:1000.
IHC analysis of FFPE human breast carcinoma tissue stained with the c-Src antibody
Confocal immunofluorescent analysis of c-Src antibody with A375 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the c-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The gene for Src is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. It is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in Src could be involved in the malignant progression of colon cancer.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this c-Src antibody.
Limitation:
This c-Src antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P12931