SIRT1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F52857
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52857-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52857-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store at 4oC for up to one month. For long term, aliquot the SIRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of lysate from mouse F9 cell line using SIRT1 antibody at 1:1000. Visualized from 80~120 kDa depending on post-translational modifications
2 / 3
Fluorescent image of A549 cells stained with SIRT1 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
3 / 3
IHC analysis of FFPE mouse brain section using SIRT1 antibody at 1:25.
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Western blot analysis of lysate from mouse F9 cell line using SIRT1 antibody at 1:1000. Visualized from 80~120 kDa depending on post-translational modifications
Fluorescent image of A549 cells stained with SIRT1 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
IHC analysis of FFPE mouse brain section using SIRT1 antibody at 1:25.

Documents

Further Information

Application Details :
IHC (Paraffin): 1:25,Immunofluorescence: 1:25,Western blot: 1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the SIRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SIRT1 is a NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 566-601 of the mouse protein was used as the immunogen for the SIRT1 antibody.
Limitation:
This SIRT1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q923E4