LGR-5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F48168
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48168-0.05ML0.05 ml£260.00
Quantity:
NSJ-F48168-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the LGR-5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of LGR-5 antibody at 1:2000 dilution. Lane 1: mouse skeletal muscle; 2: rat skeletal muscle; 3: HeLa; 4: HepG2; 5: SH-SY5Y lysate; Predicted band size : 100 kDa.
2 / 3
Western blot testing of LGR-5 antibody at 1:2000 dilution. Lane 1: HeLa lysate; 2: HepG2; 3: mouse cerebellum; 4: mouse skeletal muscle lysate; 5: SH-SY5Y; Predicted band size : 100 kDa.
3 / 3
Fluorescent confocal image of HeLa cells stained with LGR-5 antibody at 1:100. Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm.
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Western blot testing of LGR-5 antibody at 1:2000 dilution. Lane 1: mouse skeletal muscle; 2: rat skeletal muscle; 3: HeLa; 4: HepG2; 5: SH-SY5Y lysate; Predicted band size : 100 kDa.
Western blot testing of LGR-5 antibody at 1:2000 dilution. Lane 1: HeLa lysate; 2: HepG2; 3: mouse cerebellum; 4: mouse skeletal muscle lysate; 5: SH-SY5Y; Predicted band size : 100 kDa.
Fluorescent confocal image of HeLa cells stained with LGR-5 antibody at 1:100. Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm.

Documents

Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the LGR-5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues. [Wiki]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 689-719 from the human protein was used as the immunogen for this LGR-5 antibody.
Limitation:
This LGR-5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75473