SUMO2/3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F42027
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42027-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42027-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SUMO2/3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 8
Fluorescent image of SH-SY5Y cells stained with SUMO2/3 antibody diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
2 / 8
Fluorescent image of HeLa cells stained with SUMO2/3 antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
3 / 8
Fluorescent image of U251 cells stained with SUMO2/3 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
4 / 8
COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Top: lysate tested with ERM Ab. Center: IP with FLAG Ab followed by WB with NSJ# F42008 SUMO antibody. Bottom: IP with FLAG Ab followed by WB with NSJ# F42027 SUMO2/3 antibody. (*) represents immunoprecipitated ERM-like forms detected by SUMO Abs.
5 / 8
Western blot analysis of lysate from (1) 293T, (2) HeLa, (3) HL-60, (4) Jurkat cell lines and (5) rat liver tissue using SUMO2/3 antibody at 1:1000.
6 / 8
SUMO2/3 antibody western blot analysis in (10 U251 cells, (2) mouse liver and (3) rat liver lysate.
7 / 8
Western blot testing of SUMO2/3 antibody and 293T cell lysate either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO3 gene.
8 / 8
IHC testing of human hepatocarcinoma stained with SUMO2/3 antibody.
❮❯

Fluorescent image of SH-SY5Y cells stained with SUMO2/3 antibody diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Fluorescent image of HeLa cells stained with SUMO2/3 antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Fluorescent image of U251 cells stained with SUMO2/3 antibody. Ab was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Top: lysate tested with ERM Ab. Center: IP with FLAG Ab followed by WB with NSJ# F42008 SUMO antibody. Bottom: IP with FLAG Ab followed by WB with NSJ# F42027 SUMO2/3 antibody. (*) represents immunoprecipitated ERM-like forms detected by SUMO Abs.
Western blot analysis of lysate from (1) 293T, (2) HeLa, (3) HL-60, (4) Jurkat cell lines and (5) rat liver tissue using SUMO2/3 antibody at 1:1000.
SUMO2/3 antibody western blot analysis in (10 U251 cells, (2) mouse liver and (3) rat liver lysate.
Western blot testing of SUMO2/3 antibody and 293T cell lysate either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO3 gene.
IHC testing of human hepatocarcinoma stained with SUMO2/3 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25-100,Western blot: 1:1000,Immunohistochemistry (FFPE): 1:50-100
Application Note:
Titration of the SUMO2/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 49-81 from human SUMO3 was used as the immunogen for this SUMO2/3 antibody.
Limitation:
This SUMO2/3 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Species Reactivity (Predicted):
Bovine, Chicken, Hamster, Pig, Primate, Rat, Xenopus, Zebrafish
Uniprot #:
P55854