Aldolase Antibody

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NSJ Bioreagents
Product Code: NSJ-F48149
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48149-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48149-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Aldolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from 1) 293, 2) RD, 3) mouse NIH3T3 and 4) rat L6 cell line using Aldolase antibody at 1:1000. Predicted molecular weight ~40 kDa.
2 / 3
IHC analysis of FFPE human lung carcinoma tissue stained with Aldolase antibody
3 / 3
Confocal immunofluorescent analysis of Aldolase antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of lysate from 1) 293, 2) RD, 3) mouse NIH3T3 and 4) rat L6 cell line using Aldolase antibody at 1:1000. Predicted molecular weight ~40 kDa.
IHC analysis of FFPE human lung carcinoma tissue stained with Aldolase antibody
Confocal immunofluorescent analysis of Aldolase antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the Aldolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 66-95 from the human protein was used as the immunogen for this Aldolase antibody.
Limitation:
This Aldolase antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Species Reactivity (Predicted):
Rabbit
Uniprot #:
P04075