Bcl-2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F42666
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42666-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42666-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from mouse C2C12, rat PC-12 cell line using Bcl-2 antibody diluted at 1:500 for each lane.
2 / 4
IHC analysis of FFPE human testis tissue stained with Bcl-2 antibody
3 / 4
Confocal immunofluorescent analysis of Bcl-2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
4 / 4
(Left) HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using NSJ# F42666 Bcl-2 antibody for IP and a different Bcl-2 antibody for WB. (Right) FACS testing of same transfected cells with NSJ# F42666.
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Western blot analysis of lysate from mouse C2C12, rat PC-12 cell line using Bcl-2 antibody diluted at 1:500 for each lane.
IHC analysis of FFPE human testis tissue stained with Bcl-2 antibody
Confocal immunofluorescent analysis of Bcl-2 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
(Left) HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using NSJ# F42666 Bcl-2 antibody for IP and a different Bcl-2 antibody for WB. (Right) FACS testing of same transfected cells with NSJ# F42666.

Documents

Further Information

Application Details :
Western blot: 1:500,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BCL2 is integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 75-110 (BH3 binding domain) from the human protein was used as the immunogen for this Bcl-2 antibody.
Limitation:
This Bcl-2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Species Reactivity (Predicted):
Bovine
Uniprot #:
P10415