LCK Antibody

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NSJ Bioreagents
Product Code: NSJ-F50711
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50711-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50711-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the LCK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Confocal immunofluorescent analysis of LCK antibody with A2058 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
2 / 4
IHC analysis of FFPE human breast carcinoma tissue stained with the LCK antibody
3 / 4
Western blot analysis of lysate from 1) mouse thymus and 2) rat thymus tissue lysate using LCK antibody at 1:1000. Predicted molecular weight ~58 kDa.
4 / 4
LCK antibody flow cytometric analysis of A2058 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Confocal immunofluorescent analysis of LCK antibody with A2058 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human breast carcinoma tissue stained with the LCK antibody
Western blot analysis of lysate from 1) mouse thymus and 2) rat thymus tissue lysate using LCK antibody at 1:1000. Predicted molecular weight ~58 kDa.
LCK antibody flow cytometric analysis of A2058 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the LCK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
LSK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3'-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LSK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t(1;7)(p34;q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 23-52 from the human protein was used as the immunogen for this LCK antibody.
Limitation:
This LCK antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P06239