SMAD1 Antibody

NSJ Bioreagents
Product Code: NSJ-F52570
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52570-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52570-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SMAD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC analysis of FFPE human heart section using SMAD1 antibody; Ab was diluted at 1:25.
2 / 4
Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.
3 / 4
Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.
4 / 4
Western blot analysis of lysate from (1) HT-1080, (2) HUVEC, (3) mouse C2C12 cell line and (4) rat liver tissue using SMAD1 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.

IHC analysis of FFPE human heart section using SMAD1 antibody; Ab was diluted at 1:25.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle section using SMAD1 antibody; Ab was diluted at 1:25 dilution.
Western blot analysis of lysate from (1) HT-1080, (2) HUVEC, (3) mouse C2C12 cell line and (4) rat liver tissue using SMAD1 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.

Further Information

Application Details :
Immunofluorescence: 1:25,IHC (Paraffin): 1:25,Western blot: 1:1000
Application Note:
Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SMADs are intracellular proteins that transduce extracellular signals from transforming growth factor beta ligands to the nucleus where they activate downstream gene transcription. The SMADs, which form a trimer of two receptor-regulated SMADs and one co-SMAD, act as transcription factors that regulate the expression of certain genes. There are three classes of SMADs, SMAD1 belongs to the receptor-regulated class (R-SMAD) which includes SMAD2, SMAD3, SMAD5 and SMAD8/9. [Wiki]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This SMAD1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.
Limitation:
This SMAD1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Species Reactivity (Predicted):
Bovine
Uniprot #:
Q15797