DHFR Antibody

NSJ Bioreagents
Product Code: NSJ-F52556
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52556-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52556-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the DHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot analysis of lysate from 1) 293T, 2) HeLa, 3) Jurkat, 4) Y79 cell line , 5) mouse spleen and 6) rat kidney tissue lysate using DHFR antibody at 1:1000. Predicted molecular weight ~22 kDa.
2 / 3
IHC analysis of FFPE human liver section using DHFR antibody; Ab was diluted at 1:25.
3 / 3
Immunohistochemical analysis of paraffin-embedded human brain section using DHFR antibody; Ab was diluted at 1:25 dilution.

Western blot analysis of lysate from 1) 293T, 2) HeLa, 3) Jurkat, 4) Y79 cell line , 5) mouse spleen and 6) rat kidney tissue lysate using DHFR antibody at 1:1000. Predicted molecular weight ~22 kDa.
IHC analysis of FFPE human liver section using DHFR antibody; Ab was diluted at 1:25.
Immunohistochemical analysis of paraffin-embedded human brain section using DHFR antibody; Ab was diluted at 1:25 dilution.

Further Information

Application Details :
IHC (Paraffin): 1:25,Western blot: 1:1000
Application Note:
Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-50 from human Dihydrofolate reductase was used as the immunogen for this DHFR antibody.
Limitation:
This DHFR antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Species Reactivity (Predicted):
Bovine, Pig
Uniprot #:
P00374