HNF4A Antibody

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NSJ Bioreagents
Product Code: NSJ-F48198
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48198-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48198-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the HNF4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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HNF4A antibody western blot analysis in 1) 293, 2) HepG2 cell line and 3) rat liver tissue lysate.
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HNF4A antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue.
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Confocal immunofluorescent analysis of HNF4A antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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HNF4A antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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HNF4A antibody western blot analysis in 1) 293, 2) HepG2 cell line and 3) rat liver tissue lysate.
HNF4A antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue.
Confocal immunofluorescent analysis of HNF4A antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
HNF4A antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 281-312 from the human protein was used as the immunogen for this HNF4A antibody.
Limitation:
This HNF4A antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Rat
Species Reactivity (Predicted):
Mouse
Uniprot #:
P41235