SUMO2/3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F42562
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F42562-0.08ML0.08 ml£260.00
Quantity:
NSJ-F42562-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SUMO2/3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC analysis of FFPE human bladder section using SUMO2/3 antibody; Ab was diluted at 1:100.
2 / 4
Fluorescent confocal image of HeLa cell stained with SUMO2/3 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:400, 50 min at 37oC). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37oC). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). immunoreactivity is localized to the nucleus.
3 / 4
SUMO2/3 antibody western blot analysis in (1) 293 cell line and (2) rat liver tissue lysate. Observed molecular weight: 12-15 kDa.
4 / 4
IHC analysis of FFPE human hepatocarcinoma tissue stained with the SUMO2/3 antibody
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IHC analysis of FFPE human bladder section using SUMO2/3 antibody; Ab was diluted at 1:100.
Fluorescent confocal image of HeLa cell stained with SUMO2/3 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with primary Ab (1:25, 1 h at 37oC). For secondary Ab, Alexa Fluor 488 conjugated donkey anti-rabbit Ab (green) was used (1:400, 50 min at 37oC). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37oC). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). immunoreactivity is localized to the nucleus.
SUMO2/3 antibody western blot analysis in (1) 293 cell line and (2) rat liver tissue lysate. Observed molecular weight: 12-15 kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with the SUMO2/3 antibody

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:50-1:100,Western blot: 1:1000
Application Note:
Titration of the SUMO2/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This SUMO2/3 antibody recognizes SUMO2 and SUMO3. It was produced from rabbits immunized with a recombinant protein encoding full length human SUMO3.
Limitation:
This SUMO2/3 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Rat
Uniprot #:
P55854