WNT10B Antibody

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NSJ Bioreagents
Product Code: NSJ-F49724
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F49724-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49724-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the WNT10B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of WNT10B antibody and MDA-MB231 lysate. Predicted molecular weight ~45 kDa.
2 / 5
Western blot analysis of WNT10B antibody and mouse liver tissue lysate. Predicted molecular weight ~45 kDa.
3 / 5
WNT10B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma.
4 / 5
Flow cytometric analysis of MDA-231 cells using WNT10B antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
5 / 5
Western blot testing of 1) mouse skeletal muscle and 2) rat skeletal muscle lysate with WNT10B antibody. Predicted molecular weight ~45 kDa.
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Western blot analysis of WNT10B antibody and MDA-MB231 lysate. Predicted molecular weight ~45 kDa.
Western blot analysis of WNT10B antibody and mouse liver tissue lysate. Predicted molecular weight ~45 kDa.
WNT10B antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma.
Flow cytometric analysis of MDA-231 cells using WNT10B antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot testing of 1) mouse skeletal muscle and 2) rat skeletal muscle lysate with WNT10B antibody. Predicted molecular weight ~45 kDa.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the WNT10B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 193-222 from the human protein was used as the immunogen for this WNT10B antibody.
Limitation:
This WNT10B antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Rat
Uniprot #:
O00744